It is needed to elucidate the lymphatic network connecting each lymph node to establish a lymphatic drug delivery system. We succeeded in establishment of MXH10/Mo-lpr/lpr inbred mice which develop systemic swelling of LNs up to 10 mm in diameter. We identified 22 different lymph nodes situated in the head and neck, limbs, thoracic and abdominal regions. This paper unveils the anatomy of murine lymphatics to give new insights into the investigation of inter-lymph node metastasis of cancer, especially the mechanisms involved in the trafficking of cancer cells through inter-LN vessels. The results provide data that may prove very useful in the quest to develop better lymph drainage-based drug delivery systems |