Staff

• Professor
  Shin MIZUKAMI
• Associate Professor
  Toshiyuki KOWADA

Outline

* Development of chemical probes for bioimaging
* Development of photoregulation technology of biomolecular functions
* Structural biology and bioinorganic chemistry of heme-related proteins

Research Overview

In a living cell or body, various molecules such as small molecules, proteins, nucleic acids, and sugars are functioning through interaction with each other. To precisely understand their biological functions, it is important to investigate the activities or behaviors of the target molecules under living condition, in which all interactions with other biomolecules are maintained. We are studying physiological roles of target biomolecules through development of chemistry-based methods to image and regulate their functions.

Representative recent publications

• Intracellular protein-labeling probes for multicolor single-molecule imaging of immune receptor-adaptor molecular dynamics
  R. Sato, J. Kozuka, M. Ueda, R. Mishima, Y. Kumagai, A. Yoshimura, M. Minoshima, S. Mizukami, K. Kikuchi
  J. Am. Chem. Soc. 2017, 139, 17397–17404.
• Visualization of Long-term Mg2+ Dynamics in Apoptotic Cells with a Novel Targetable Fluorescent Probe
  Y. Matsui, Y. Funato, H. Imamura, H. Miki, S. Mizukami, K. Kikuchi
  Chem. Sci. 2017, 8, 8255-8264.
• Enzyme-Triggered Compound Release using Functionalized Antimicrobial Peptide Derivatives
  S. Mizukami, M. Kashibe, K. Matsmoto, Y. Hori, K. Kikuchi
  Chem. Sci. 2017, 8, 3047-3053
• Real-time Intravital Imaging of pH Variation Associated with Osteoclast Activity
  H. Maeda, T. Kowada, J. Kikuta, M. Furuya, M. Shirazaki, S. Mizukami, M. Ishii, K. Kikuchi
  Nat. Chem. Biol. 2016, 12, 579–585.
• Unique Coupling of Mono- and Dioxygenase Chemistries in a Single Active Site Promotes Heme Degradation
  T. Matsui, S. Nambu, C. W. Goulding, S. Takahashi, H. Fujii, M. Ikeda-Saito
  Proc. Natl. Acad. Sci. U.S.A. 2016, 113, 3779–3784.
• Activatable ¹⁹F MRI Nanoparticle Probes for the Detection of Reducing Environments
  T. Nakamura, H. Matsushita, F. Sugihara, Y. Yoshioka, S. Mizukami, K. Kikuchi
  Angew. Chem. Int. Ed. 2015, 54, 1007–1010.
• Multifunctional Core-shell Silica Nanoparticles for Highly Sensitive ¹⁹F Magnetic Resonance Imaging
  H. Matsushita, S. Mizukami, F. Sugihara, Y. Nakanishi, Y. Yoshioka, K. Kikuchi
  Angew. Chem. Int. Ed. 2014, 53, 1008–1011.
• Small-Molecule Based Protein-Labeling Technology in Live Cell Studies: Probe-Design Concepts and Applications
  S. Mizukami, Y. Hori, K. Kikuchi
  Acc. Chem. Res. 2014, 47, 247–256.

Contact Information

“Please delete “/” after “@” in a mail address.”
Shin MIZUKAMI
(TEL&FAX +81-22-217-5116, Email: s-mizu@/tagen.tohoku.ac.jp)
Toshiyuki KOWADA
(TEL&FAX +81-22-217-5118, Email: tkowada@/tagen.tohoku.ac.jp)